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Sterility Testing – Biological Indicators

Biological indicators (BIs) are carriers, such as a paper strip, that are inoculated with a specified level of a particular organism (typically Bacillus species). BIs are used to validate and/or monitor certain sterilization

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Spore strips are transferred from their primary package to Soybean Casein Digest Medium (SCDM) and incubated for the recovery of the indicated organism. BI occurs through three different methods: direct transfer, within product, or self-contained in the product or packaging.